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Effects of macrophage on unloading/reloading‐related regulation of muscle fiber size in op/op mice
Author(s) -
Ohira Takashi,
Wang Xiao Dong,
Kawano Fuminori,
Terada Masahiro,
Yamamoto Hiroshi,
Ito Tsunekata,
Nakai Naoya,
Goto Katsumasa,
Ohira Yoshinobu
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.601.9
Subject(s) - macrophage , soleus muscle , transplantation , atrophy , muscle atrophy , andrology , medicine , endocrinology , biology , chemistry , microbiology and biotechnology , immunology , skeletal muscle , biochemistry , in vitro
Roles of macrophages in the regulation of soleus muscle fibers of adult op/op (‐/‐) mice, without the macrophage colony‐stimulating factor, were studied. Mice were hindlimb‐suspended for 10 days and some mice were allowed to recover in the cage for additional 10 days. Macrophages, obtained from heterozygous () mice were transplanted into half of the recovery group i.p. immediately after termination of unloading. The samplings of soleus muscle were performed before suspension, immediately after suspension, and 10 days after recovery. The unloading‐related fiber atrophy in ‐/‐ mice without macrophage transplantation did not recover following 10 days of reloading, whereas that in the wild type and mice were increased to the control level. That in ‐/‐ mice was recovered by transplantation of macrophages. These morphological responses were closely related to the changes in the number of myonuclei and the quiescent and mitotic active satellite cells. It is suggested that the macrophages play one of the key roles in the regulation of muscle fiber size in mice.