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Differential proteome profiling analysis of male and female murine skeletal muscle following a single bout of exercise
Author(s) -
Gan Yutian,
Kulp Mohini,
Smith Laura,
Scordilis Stylianos P
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.601.5
Subject(s) - proteome , skeletal muscle , creatine kinase , heat shock protein , myofibril , endocrinology , medicine , hsp70 , biology , lactate dehydrogenase , treadmill , protein expression , fold change , andrology , gene expression , enzyme , biochemistry , gene
Identifying the array of proteins that change expression following a single bout of eccentrically‐biased exercise in skeletal muscle will shed light on the molecular mechanisms underlying adaptation, as well as their gender specificity. Exercise‐naive male and female mice (C57BL/10ScSnJ 000476 +/+) ran downhill (‐15°) on a treadmill at 25 m s −1 for 15 min and 800 ug of total biceps brachii extract was electrophoresed on pH 5‐8 2‐D gels. The resulting spots that changed at least +/‐ 2‐fold and were significantly different (p<0.05) relative to unexercised controls of the appropriate gender using PDQuest 8.0 were analyzed by LC/MS‐MS and identified using BioWorks 3.3.1. This analysis showed significant proteome remodeling subsequent to downhill running. The expression change patterns were followed at 0, 24, 48, 72 and 168 hrs post‐exercise (PX) and significant changes were observed in 4 NCBI KOG groups. Both myofibrillar and cytoskeletal protein remodelings were noted, as well as decreased expression of creatine kinase. Nearly all of the glycolytic enzymes were up‐regulated. The cellular heat shock protein responses were significant and specific. Roughly the same number of protein spots changed in females and males, but the females peaked at 24 hr PX, whereas in males the peak was at 72 hr PX. Supported by NSF 0420971, the Smith College Blakeslee Fund and HHMI.