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heparin inhibits injury‐induced femoral artery remodeling in mouse Via CD44
Author(s) -
Zhao Gaofeng,
Kolliputi Narasaiah,
Zhao Hang,
Beagle John,
Yu Lunyin,
Syrkina Olga,
Leu Shawwei,
Hicham Mrabat,
Hales Charles
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.592.8
Subject(s) - heparin , restenosis , femoral artery , medicine , artery , cd44 , stenosis , inflammation , chemistry , stent , biochemistry , cell
Background Previous studies have indicated heparin which has both anti‐coagulant and anti‐proliferative properties, may be used as a possible therapeutic agent for the treatment of post‐angioplasty restenosis. CD 44 is the receptor of hyaluronan and plays essential roles in many aspects including SMC proliferation, inflammation and injury repair. This study investigates the effects of heparin on injury‐induced femoral artery remodeling and the expression of CD 44 , to illuminate the molecular mechanism by which heparin blocks injury‐induced femoral artery stenosis. Hypothesis Heparin decreases injury‐induced femoral artery remodeling through the CD 44 signal pathway, Methods The injury‐induced artery remodeling model was prepared in wild‐type and CD 44 knock‐out mice by inserting a straight spring wire into the femoral artery via arterioctomy in a small muscular branch. Then mice were treated with heparin by subcutaneous injection at a dose of 20mg/kg once a day. 4 weeks later, we sacrificed the mice for assessment of histology of the injured femoral artery and for the expression of CD44. Results In wild‐type mice, heparin significantly decreased femoral artery remodeling, and western blot showed heparin could markedly promote CD 44 expression in the injured arteries. In contrast with wild‐type mice, CD 44 deficient mice demonstrated considerable greater arterial remodeling, which when treated with heparin, no longer showed any significant reduction in vascular remodeling. Conclusion Heparin can decrease injury‐induced femoral artery remodeling, at least partially, by upregulating the expression of CD 44 .