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Glutathione levels in ischemia‐induced angiogenesis
Author(s) -
Nijmeh Julie,
Moldobaeva Aigul,
Wagner Elizabeth
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.592.5
Subject(s) - glutathione , angiogenesis , chemistry , reactive oxygen species , ischemia , acetylcysteine , antioxidant , pharmacology , oxidative stress , neovascularization , biochemistry , medicine , enzyme
We previously have shown, using fluorescent indicators, increased levels of reactive oxygen species (ROS) in a mouse model of lung angiogenesis following left pulmonary artery ligation (LPAL). Furthermore, we demonstrated that ROS play an important role in promoting ischemia‐induced angiogenesis in that treatment with the antioxidant N‐acetylcysteine (NAC) showed reduced neovascularization. In this study, we measured levels of reduced glutathione (GSH), the most abundant antioxidant, and its oxidized form (GSSG) in the lungs as an indicator of ROS levels early after LPAL. Additionally, we assessed the effect of NAC on GSH/GSSG following LPAL. Mice were treated with NAC (ip; 1mg/g body wt), or vehicle before LPAL (‐24hrs, ‐12hrs, 0hr), or sham. After surgery, lungs were harvested (30min, 4hrs, 24hrs) and processed for measuring GSH/GSSG (Oxis kit). Lungs following 4hrs LPAL showed a significant 44% reduction in GSH/GSSG levels compared to sham. NAC treated lungs, however, had an increased baseline GSH/GSSG that did not change with LPAL. These GSH/GSSG measurements confirmed early ROS release after LPAL and further emphasized the pivotal role of ROS ischemia‐induced angiogenesis.