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Temporal and Spatial Activation of Angiogenesis and Expression of FGF‐1/FGF Receptors in the Infarcted Heart
Author(s) -
Zhao Tieqiang,
Zhao Wenyuan,
Chen Yuanjian,
Sun Yao
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.592.2
Subject(s) - angiogenesis , fibroblast growth factor , immunostaining , receptor , medicine , endocrinology , immunohistochemistry
The current study is to determine the temporal and spatial expression of FGF‐1/FGF receptors and their relation to angiogenesis in the infarcted heart. Rat hearts were collected at day 1, 3, 5, 7, 14, 21 and 28 postMI. Normal rats served as controls. Cardiac FGF‐1 gene and protein expression, FGF receptors and vascular density were examined. Pre‐existing vessels underwent necrosis in the infarcted myocardium at day 1 postMI. Newly formed vessels first appeared at the border zone as early as day 3, followed by the infarcted myocardium. Vascular density peaked at day 7 and 14, and gradually declined at day 21 and 28. FGF‐1 immunostaining was strongly positive at the border zone at day 3 and at the infarcted myocardium at day 7 and 14 and then became less evident at day 28. Autoradiographic FGF receptor binding was significantly increased in the infarcted myocardium from day 7 and remained elevated thereafter. The expression of FGF‐1 and FGF receptors remained unchanged in noninfarcted myocardium. Thus, angiogenesis is activated in the infarcted myocardium in the early stage of MI and becomes quiescent thereafter. FGF‐1/FGF receptor expression is elevated in the infarcted myocardium, which is temporally and spatially coincident with angiogenesis. This finding suggests that FGF‐1 is involved in cardiac angiogenesis following MI.