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The effect of morphine on a K+ channel from a murine enteric neuron cell line derived from the H‐2kb‐tsA58 mouse
Author(s) -
Hawkins Gregory,
Dewey William,
Srinivasan Shanthi,
Akbarali Hamid
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.580.3
Subject(s) - pipette , patch clamp , cell culture , morphine , chemistry , immunocytochemistry , electrophysiology , ion channel , biophysics , opioid , microbiology and biotechnology , hepes , inhibitory postsynaptic potential , transmembrane protein , neuroscience , receptor , pharmacology , biology , endocrinology , biochemistry , genetics
Historically the generation and study of isolated primary murine enteric neurons (EN) has proven extremely difficult. Recently an EN cell line has been developed from the H‐2kb‐tsA58 mouse (immortomouse). In the present study we examined ion channel expression in these ENs. Single channel recordings were made by cell attached patch clamp with high K+ (140mM) in the bath and KCl (140mM), HEPES (10mM) in the pipette in order to zero the transmembrane potential. We have identified an outward current with average amplitude of 3.8 pA at +40mV. The conductance of this channel was 10.2 pS and was blocked by 130mM Cs+ in the pipette solution. Application of 1 µM morphine in the bath decreased the open probability of this channel from 0.01945 to 0.00634. Expression of µ‐opioid receptors were detected by immunocytochemistry. Our findings demonstrate that EN cells express a K+ channel that is sensitive to morphine and which may play an important role in the study of opioid bowel disorders.