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The Morphological Investigation Of The Mechanism Of Astragalus Polysaccharide Against Endoplasmic Reticulum Stress By Activation Of AMPK In TIID Rats
Author(s) -
Zhao Ming,
Ouyang Jingping
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.578.8
Subject(s) - ampk , endoplasmic reticulum , endocrinology , medicine , astragalus , panax notoginseng , immunohistochemistry , rat model , chemistry , phosphorylation , protein kinase a , biochemistry , pathology , traditional chinese medicine , alternative medicine
OBJECTIVE To investigate the effects of Astragalus polysaccharide on reducing ER stress in a rat Model of Type 2Diabetes. Methods 44 SD male rats were randomly divided into two groups, group A was fed with high fat forage and injected with small dosage STZ 25mg/kg through caudal vein, successful rat models were randomly divided into two groups: diabetes group DM group,DM +APS group.Group B were fed with normal forage and also randomly divided into two groups: control group (group C), control+APS (group C +APS group) ,C +APS group and DM +APS group were administered with APS 700mg/kg/day , while group C and DM group were administered with the same dose of Sodium Chloride for 8 weeks. The animals were killed at the end of 8 week treatment with APS,the expression of p‐PERK and p‐AMPK in liver tissue was detected by immunohistochemistry. Results 1, The expression of p‐PERK was significantly increased in DM group as compared with the control group ,but the expression in DM +APS group was markedly lower than DM group 2,The expression of p‐AMPK was significantly decreased in DM group as compared with the control group, but the expression in DM group was markedly lower than DM+APS group. Conclusion APS treatment can relieve ER stress through reducing the expression of phosphorylated PERK in a rat model of type 2 diabetes by activation of AMPK.