Premium
Curcumin protects against glucose‐induced endothelial dysfunction through HO‐1 and GC pathway
Author(s) -
Fang Xiaodong,
Yang Fan,
Zhu Li,
Shen Yueliang,
Chen Yingying
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.578.2
Subject(s) - curcumin , methylene blue , vasodilation , chemistry , heme oxygenase , pharmacology , endothelium , acetylcholine , heme , contraction (grammar) , thoracic aorta , endocrinology , medicine , aorta , biochemistry , enzyme , photocatalysis , catalysis
Aim To investigate the effect and mechanism of curcumin on high glucose induced injury in blood vessels. Methods The thoracic aortic rings with or without endothelium from male SD rats were mounted in an organ bath. Isometric contraction of aortic rings was measured. Heme oxygenase (HO) activity was also evaluated. Results (1)After incubation with 44 mmol/L of high glucose for 2 h, the vascular relaxation response to acetylcholine (Ach) decreased in an endothelium‐dependent manner; (2)Coincubation with curcumin (10 −13 ~10 −11 mol/L) and high glucose, the high glucose‐induced vasodilation dysfunction was inhibited in a dose‐dependent manner; (3) Curcumin increased the HO activity of thoracic aorta. ZnPPIX (an inhibitor of heme oxygenase‐1) offset the protective effect of curcumin. ; (4) A non‐selective guanylate cyclase (GC) inhibitor methylene blue also completely abolished the protective effect of curcumin. Conclusion Curcumin could alleviate the high glucose‐induced acute endothelium‐dependent vasorelexation dysfunction in rat aortic rings. HO‐1 and GC were proposed as a mechanism to account for the protection of vascular responses by curcumin.