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Elevated Advanced Glycation Endproducts (AGEs) in Rat Models
Author(s) -
Zhao Chunsheng,
Yang Jieying,
Zhang Yan,
Tan Ningzhi,
Zhu JiaShi
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.578.15
Subject(s) - medicine , endocrinology , glycation , fructose , diabetes mellitus , sucrose , alloxan , chemistry , biochemistry
It is reported that plasma AGEs are elevated in patients with diabetes mellitus (DM) and in rats with STZ‐induced DM, where non‐enzymatic formation of AGEs causes crosslink of proteins, lipids and DNA. We examined in this study the plasma AGEs fluorescence (ex. 360nm, em. 450nm) in 3 rat models. (1) Male SD rats were given STZ (i.p. 60mg/kg), and continuously drank water or 5% fructose or sucrose water. Addition of fructose in drinking water caused higher mortality (92%) in rats, compare to controls (p<0.001). Addition of sucrose did not increase plasma AGEs in STZ‐induced DM rats (p>0.05). (2) In alloxan‐induced (i.v. 40mg/kg) DM rats, plasma AGEs were increased by 41%, 108% and 239% at Wks 4, 8 and 12, respectively, compared to normal rats (p<0.05). Carboxymethyl lysine (CML) and HbA1c were also increased at Wk 8 by 673% and 239%, respectively (p<0.01). (3) In a high‐calorie diet‐induced Metabolic Syndrome model in male SD rats, plasma AGEs were increased by 12% at Wk 12, as compared to normal rats (p<0.05). In summary, the alloxan‐induced DM rats and diet‐induced Syndrome X rats may serve as animal models for AGEs research, in addition to the STZ model. Fructose or sucrose drinks are not options of increasing plasma AGEs in the STZ‐induced DM rats. It is to be studied if fructose drinks would accelerate plasma AGEs elevation in diet‐induced Syndrome X rats.