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Photobiomodulation reduces perilesional myocardial injury during radiofrequency ablation in guinea pig hearts
Author(s) -
Henry Michele M,
Stowe David F,
Heisner James S,
Rao Rohit,
Dhala Anwar,
Camara Amadou KS,
Whelan Harry T
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.576.8
Subject(s) - ablation , perfusion , lesion , irradiation , guinea pig , radiofrequency ablation , nuclear medicine , biophysics , medicine , chemistry , materials science , pathology , cardiology , biology , physics , nuclear physics
Radiofrequency (RF) ablation of arrhythmogenic cardiac lesions invariably injures normal tissue beyond the intended pathological territory requiring therapy. We tested if the unintended area of tissue damage could be restricted in the presence of long‐wavelength, low‐intensity photon irradiation (LLPI) in the far‐red, near‐infrared (NIR) spectra. Guinea pig hearts were isolated and perfused with Krebs‐Ringer's solution at 37°C. Lesions were induced on the left ventricular (LV) surface using a 2 mm tip diameter probe (Ablation Controller EPT‐1000XP) for exactly 1 min at 15 W (probe tip maximum 55‐58 °C) in the absence and presence of NIR (670 nm) light emitted (output 1300 mW/cm 2 ) from a 1 cm diameter light‐emitting diode (LED) array placed 4 mm from the LV surface. NIR light was applied for 15 min immediately after induction of the lesion. One hour later perfusion was stopped and hearts were removed and prepared for sectioning and staining. We found that the surface area and depth of lesions, i.e. volume, was reduced by more than 50% when NIR light was applied. NIR light application may help to retain mitochondrial cytochrome oxidase activity in heat damaged, but not destroyed, cells in the perimeter about the lesion. LLPI during RF ablation may increase its precision by restricting damage to the intended area. Supported by NASA, NIH, AHA and VA Merit grants.