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Cardiac ischemic/reperfusion injury in diabetic rats treated with a superoxide dismutase mimetic
Author(s) -
Schwartz Dean D,
Carson Melissa,
Rogers Colin B,
Judd Robert L,
Plaisance Eric P
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.576.5
Subject(s) - streptozotocin , contractility , medicine , ischemia , lipid peroxidation , superoxide dismutase , cardiac function curve , diabetes mellitus , reperfusion injury , cardiology , myocardial infarction , oxidative stress , anesthesia , pharmacology , endocrinology , heart failure
The diabetic heart is at an increased risk for myocardial infarction. The aim of this study was to examine the effect of the superoxide dismutase mimetic EUK‐134 on the susceptibility of the diabetic heart to ischemic/reperfusion injury. Rats were injected with streptozotocin (STZ) or its vehicle (Control) and implanted with an osmotic minipump containing the SOD mimetic EUK‐134 or its vehicle. After 4 weeks, in vivo left ventricular (LV) pressure and contractility were similar in all four groups. Hearts were then isolated and perfused in the Langendorf mode for the measurement of LV pressure and contractility before and after a 30 min global ischemia. Baseline LV pressure was similar in all groups. Following 30 min of ischemia, LV pressure was significantly reduced in the control rats treated with vehicle whereas hearts from STZ rats treated with vehicle or EUK‐134 and control rats treated with EUK‐134 all recovered more quickly and regained normal mechanical function following ischemia/reperfusion. Heart lipid peroxidation was also increased in the vehicle‐treated control rats compared to the other groups. These results suggest that hyperglycemia associated with STZ diabetes and/or treatment with the SOD mimetic EUK‐134 reduce cardiac lipid peroxidation and enhance cardiac mechanical activity after ischemia/reperfusion.