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Involvement of H2O2 in benzo[a]pyrene‐induced proliferation in mouse aortic endothelial cells.
Author(s) -
Jules George E.,
Onumah Ogabeyalu E.K.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.576.4
Subject(s) - cell growth , angiogenesis , benzo(a)pyrene , flow cytometry , catalase , chemistry , reactive oxygen species , intracellular , cell cycle , apoptosis , cell , microbiology and biotechnology , biochemistry , biology , carcinogen , cancer research , enzyme
Benzo[ a ]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), has been shown to elevate intracellular reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ) and superoxide. It has been well established that low concentrations of H 2 O 2 promote proliferation in various cell types, including endothelial cells (ECs). Proliferation of ECs is a critical step of angiogenesis, which is required for a number of normal biological processes, such as normal tissue development and wound repair. Angiogenesis is also involved in several human diseases such as cancer and the development of atherosclerotic plaques. The central hypothesis to be tested in this proposal is that the generation of H 2 O 2 is a mechanism by which BaP promotes mouse aorta endothelial cell (MAEC) proliferation. Cell proliferation assays and flow cytometry analyses were used to determine the effect of BaP on the proliferation rate of MAECs isolated from mice overexpressing human catalase ( hCat Tg) and their wild‐type littermates. Results suggest that BaP promotes proliferation of ECs isolated from the aorta of wild‐type mice. However, the molecular mechanism by which BaP promotes MAEC proliferation is not fully understood. Catalase is an enzyme that converts H 2 O 2 to water and oxygen. BaP‐treated hCat Tg MAECs show a reduced level of proliferation when compared to wild‐type MAECs, in addition to changes in the activity and expression of proteins that regulate cell proliferation and cell cycle progression.