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Protective effects of the N‐(2‐Mercaptopropionyl)‐Glycine and N‐acetylcysteine on cigarette smoke‐induced lung oxidative stress in mice
Author(s) -
Valenca Samuel S,
Bezerra Frank S,
Pires K MP,
Lanzetti Manuella,
Zin Walter A,
Porto Luis Cristovao
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.572.6
Subject(s) - acetylcysteine , oxidative stress , chemistry , glutathione , pharmacology , antioxidant , glycine , smoke , medicine , biochemistry , enzyme , amino acid , organic chemistry
Cigarette smoke (S) causes damage in lung by oxidants/antioxidants imbalance. We investigated the protective effects of allopurinol (APN), N‐(2‐mercaptopropionyl)‐glycine (MPG) and N‐acetylcysteine (NAC) on both acute and chronic S exposure by histological and biochemical methods. C57BL/6 mice were exposed to 6 S/day during 5 days (S5d) or 12 S/day during 60 days (S60d) and treated with 50 mg/kg/day of APN (S5d+APN and S60d+APN), 60 mg/kg/day of MPG or NAC (S5d+MPG, S60d+MPG, S5d+NAC and S60d+NAC). Mice exposed to ambient air were used as controls (A5d and A60d). BAL counts of alveolar macrophages (AM) and neutrophils (PMN) were higher in S5d and S60d compared with A5d and A60d (p<0.001). The AM and PMN in BA of S5d+APN, S5d+MPG and S5d+NAC were lower when compared with S5d (p<0.001) and similar to A5d. The AM and PMN in BAL of S60d+MPG and S60d+NAC were increased in comparison with A60d, but decreased when compared to S60d. The SOD, CAT and GSH‐Px activities in lung homogenates were increased in S5d but were significantly decreased in S60d compared to A5d and A60d. The SOD in S5d+APN, S5d+MPG and S5d+NAC were increased when compared with A5d and S5d (p<0.001). The GSH‐Px in S5d+NAC were higher when compared with S5d (p<0.01). The SOD and GSH‐Px activities in S60d+NAC were not different from A60d. MPG and NAC may be favorable antioxidant drugs during S‐induced lung oxidative stress in the mouse. Supported by FAPERJ and CNPq.