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Immunological effects of polyphenols contained in Fermented Grape Marc (FGM) on human healthy peripheral blood cells
Author(s) -
Jirillo Emilio,
Marzulli G.,
Jirillo F.,
Tafaro A.,
Kaneko M.,
Kumazawa Y.,
Amati L.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.571.7
Subject(s) - peripheral blood mononuclear cell , intracellular , cd14 , cytokine , chemistry , tumor necrosis factor alpha , ethanol , immune system , biology , immunology , biochemistry , in vitro
The effects of polyphenols extracted from FGM of Negroamaro and Koshu wines were evaluated on human Peripheral Blood Mononuclear Cells (PBMC) in terms of regulatory, inflammatory and anti‐inflammatory cytokine production. Lyophilized FGMs were suspended in water or ethanol (100 mg/ml) and supernatants were added to 1 x 10 6 PBMC/ml in culture media. After 4 hours incubation at 37°C, cells were marked with anti‐human CD4 and CD14 moAbs, respectively . To study intracellular cytokine profile, cells were permeabilized and marked with anti‐human IL‐10, IL‐12 and TNF‐α moAbs, respectively. The analysis with the flow cytometer revealed that Koshu FGM markedly enhanced intracellular content of IL‐10 by CD4 + T cells when it was suspended in water but not in ethanol. On the other hand, Koshu greatly increased the enhanced intracellular of IL‐12 by CD14 + cells (monocytes) when it was suspended in ethanol but not in water. Negroamaro FGM was able to augment the intracellular content of TNF‐α by CD14 + cells, when it was suspended in water but not in ethanol. On the contrary, Koshu markedly enhanced the intracellular content of TNF‐α when it was suspended in ethanol but not in water. These results may represent a starting point for the use of FGM as modulators of the immune response in different pathologies as well as anti‐inflammatory agents via release of IL‐10 (e.g., Koshu suspended in water). This work was in part supported by MIUR and MIN. SALUTE (Rome, Italy).