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Salmonella enterica serovar Typhimurium flagellin modulates CD4+ T cell apoptosis in Peyer's patches and spleen
Author(s) -
Fournier Benedicte Marie,
Gewirtz Andrew,
Williams Ifor,
Neish Andrew
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.570.19
Subject(s) - flagellin , microbiology and biotechnology , apoptosis , salmonella enterica , biology , mesenteric lymph nodes , spleen , tlr5 , salmonella , t cell , enterocolitis , pathogen , immunology , bacteria , immune system , medicine , innate immune system , biochemistry , genetics , toll like receptor , gastroenterology
Salmonella enterica serovar Typhimurium, a Gram‐negative pathogen, causes human gastroenterocolitis. In this study, we examined the role of flagellin in the apoptosis of Peyer's patch (PP) cells during Salmonellosis. Apoptosis is a crucial mechanism that eliminates cells without releasing noxious components. We used both enterocolitis and systemic typhoid fever models in C57BL/6 mice, with various isogenic Salmonella strains. In the enterocolitis model, an aflagellate C − B − mutant increased apoptosis of PP CD4 + T cells at 12 h post‐infection compared to the wild‐type strain, whereas the aflagellate mutant slightly decreased cell death at 24 h. In the typhoid model, the aflagellate mutant modulated CD4 + T cell apoptosis differently in PP and spleen on day 4 post‐infection. As ICAM‐1 is a costimulatory molecule involved in T cell activation, we examined whether the expression of this adhesion molecule was modified in the absence of flagellin. Interestingly, the aflagellate mutant modified ICAM‐1 expression on macrophages only in PPs but not in mesenteric lymph nodes (MLN). We also observed that the absence of flagellin elicits an increase of macrophages in PPs and MLN as well as an increased bacterial load in these organs. In conclusion, flagellin seems to be involved in the apoptosis of CD4 + T cells in several organs. However, its role appears to depend on the model, the organ and the stage of the infection.