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Coagulation profiling of class B vendor cats with reference to serine protease inhibitors
Author(s) -
McGeehan Elizabeth,
Wielgus Karen,
Azarafrooz Farshid,
Cera Lee,
Buesing Rose,
Fareed Jawed
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.569.12
Subject(s) - cats , partial thromboplastin time , thrombin time , antithrombin , coagulation , medicine , chemistry , pharmacology , heparin
Although cats from class B vendors are commonly used in biomedical research, their hematologic and blood chemistry parameters can vary widely based on health status. It was hypothesized that health status of these cats can also impact their hemostatic system. Citrated blood samples were drawn from four groups (total n=42) of class B vendor cats (R and R Research, Howard City, MI), centrifuged to obtain plasma and profiled for prothrombin time (PT), partial thromboplastin time (aPTT), Heptest, and thrombin time (TT). Anti‐Xa, anti‐IIa, heparin cofactor II (HCII), and antithrombin (AT) activities were measured using chromogenic substrate methods. Ten clinically healthy cats, habituated at a veterinary research facility for 6 months, were used as a control group. The class B vendor cats did not show variation in the PT and TT assays and only weak anti‐Xa activity (<10%).There was wide variation in the aPTT (12.2 to 49.9 sec) and Heptest (20.8 to 51.7 sec) assays. The HCII activities also showed wide variations which were largely dependent on the individual groups (range = 50%‐150%). In the AT assay similar trends were noted. These studies suggest that in contrast to conditioned cats, the class B cats exhibited wide variations in their coagulation parameters and endogenous inhibition of coagulation. Class B vendor cats may not be optimal for cardiovascular research due to variations in the hemostatic parameters.