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Dietary proteins alter hepatic and intestine lipid content and gene expression in Sprague‐Dawley rats
Author(s) -
Simmen Frank A,
Mercado Charles P,
Kang Ping,
Greenway Amy D
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.563.14
Subject(s) - jejunum , medicine , endocrinology , leptin , duodenum , small intestine , casein , biology , lipid metabolism , gene expression , soy protein , biochemistry , gene , obesity
Dietary soy protein (SPI) is anti‐obesigenic in rats; we hypothesized an intermediary role for small intestine and liver in this action. This study examined tissue lipid content and gene expression in young adult male Sprague‐Dawley rats, lifetime‐fed diets containing casein (CAS; control), SPI, or CAS supplemented with genistein (soy isoflavone; 250 mg/kg diet) (GEN). At 48 days of age, retroperitoneal fat weight was reduced in SPI vs. CAS and GEN groups; serum leptin values also were reduced with SPI. Duodenums and jejunums had more lipid content (Oil Red O staining) than did ileums and colons. SPI reduced lipid content of duodenum, jejunum and liver; GEN was without effect. In duodenum and jejunum, mRNA expression of the lipogenic enzyme genes, Fasn and Me1, was unaffected by diet. In liver, SPI suppressed Fasn and Me1 transcripts; GEN partially recapitulated these effects of SPI. In intestine, expression of the lipid catabolism‐related transcripts PPARα, Cpt1α, and Ucp2 was unaffected by diet; however, SPI (but not GEN) elevated expression of these mRNAs in liver. GEN, but not SPI, induced Cyp4a10 and suppressed Acot2 mRNAs in jejunum. Results identify lipid metabolic genes that are regulated in a complex tissue‐specific fashion by dietary factors and which may contribute to obesity. Supported by CRIS 6251‐51000‐005‐03S