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Dietary Supplementation with Dried Plum Prevents Ovariectomy‐Induced Bone Loss in C57BL/6 Mice and Modulates the Immune Response
Author(s) -
Rendina Elizabeth,
Lim Yin F.,
Marlow Denver,
Wang Yan,
Clarke Stephen L.,
Kuvibidila Solo,
Lucas Edralin A.,
Smith Brenda J.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.563.13
Subject(s) - ovariectomized rat , endocrinology , medicine , bone remodeling , splenocyte , immune system , bone mineral , bone density , stimulation , osteoporosis , ex vivo , in vivo , chemistry , biology , estrogen , immunology , spleen , microbiology and biotechnology
Due to recent evidence demonstrating the link between the immune system and skeletal health, this study was designed to determine the osteoprotective effects of dried plum (DP) on bone metabolism and the immune response. Twelve‐week old female mice (n=59) were assigned to 5 treatment groups for a duration of 4 wks: sham‐operated (Sham) control diet (AIN‐96M), ovariectomized (OVX)‐control diet and OVX animals receiving diet supplemented with 5%, 15%, or 25% DP (w/w). Bone mineral density and content of the spine was decreased (8%) by OVX, which was totally prevented by 25% DP. µCT analyses revealed the OVX compromise in spine and proximal tibial bone volume was prevented by higher doses of DP. Similar results were also observed in the trabecular number and thickness of the spine. Simulated biomechanical tests (finite element analyses) showed the improved structural properties observed with DP (15% & 25%) resulted in enhanced bone strength and stiffness. These alterations in bone mass and architecture coincided with increased serum IGF‐I and reduced bone turnover (i.e. serum PINP) in OVX mice consuming DP. Following ex vivo Con A stimulation, splenocyte TNF‐α production was suppressed by DP, but no change in IL‐6 occurred. This study supports DP's positive effects on bone in the OVX mouse model; however the role of the immune system in this response warrants further investigation. (Supported by California Dried Plum Board)Grant Funding Source California Dried Plum Board