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New screening for photoprotective compounds using zebrafish as a vertebrate model. Validation with PPL‐240
Author(s) -
RodriguezMartin Ivan,
AraujoBazan Lidia,
Palacios Lucia,
Guinea Joaquin,
Reyes Eduardo,
Gonzalez Salvador,
Acebo Paloma
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.562.2
Subject(s) - zebrafish , danio , photoprotection , model organism , photoaging , biology , trolox , microbiology and biotechnology , chemistry , biochemistry , antioxidant , genetics , photosynthesis , gene , dpph
Skin overexposure to UVR leads to photodamage (sunburn, photoaging, immune suppression and skin cancer). Cells culture models have been mostly used to perform studies of radiation cytotoxicity; however, the response of cells under culture conditions does not reflect the response of a whole organism. Zebrafish ( Danio rerio ) has increased its importance as a vertebrate model both for toxicology assays and screening of therapeutic agents, due to its transparency, fast development and easiness to handle. Using this zebrafish model, a new screening for photoprotective compounds has been developed. The photoprotective effect of Polypodium leucotomos extract (PPL‐240) has been investigated besides Trolox and vit C. Zebrafish embryos were exposed to UVB radiation, with or without previous incubation with the compounds. Irradiated embryos treated with the different compounds were compared with those untreated by evaluating the morphological abnormalities and viability up to 48 hpf. Our results, showing an increase in the viability and a decrease in the number of malformed embryos, of the PPL‐240 and Trolox treated irradiated embryos, seem to validate the use of zebrafish model to determine the effects of UVB radiation in terms of oxidation and photodamage. This new approach provides a useful, rapid and easily to perform method to detect photoprotection properties of natural extracts and other compounds.