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Enrichment of broccoli and tomato with bioactive components: effects on bioactivity markers in vivo
Author(s) -
Volker Sonja E.,
Liu Ann G.,
Erdman John W.,
Jeffery Elizabeth H.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.561.2
Subject(s) - sulforaphane , lycopene , chemistry , antioxidant , carotenoid , food science , glucoraphanin , cruciferous vegetables , reductase , in vivo , glutathione reductase , biochemistry , glutathione peroxidase , enzyme , glucosinolate , biology , botany , brassica , catalase , microbiology and biotechnology , genetics , cancer
Several bioactive components isolated from cruciferous vegetables and tomatoes exhibit anti‐cancer properties. To evaluate their bioactivity within the vegetable matrix, male Copenhagen rats were fed diets supplemented with 10% broccoli or tomato powders containing different levels of bioactives for 7 days. Indole‐3‐carbinol‐rich broccoli increased hepatic ethoxyresorufin‐O‐deethylase (EROD) and quinone reductase activity (QR) 1.4 and 1.3‐fold, respectively, and colon QR 1.6‐fold. Sulforaphane‐rich broccoli sprouts increased QR 1.9‐fold in liver and 2.3‐fold in colon, but showed no EROD induction. Selenium‐rich broccoli induced hepatic EROD 1.8‐fold; QR was elevated 2.2 and 1.8‐fold in liver and colon, respectively. No changes in EROD or QR were observed with low or high‐carotenoid, or high‐lycopene tomato diets. Serum testosterone and steroid 5‐alpha reductase 1 and 2 mRNA did not change with any dietary treatment. High‐lycopene tomato and regular broccoli diets decreased glutathione S‐transferase P1 (GSTP1) mRNA. These data show that enrichment of broccoli, but not tomato, with bioactive components enhances detoxification enzymes, whereas both vegetables decrease expression of GSTP1, a biomarker of chemopreventive action. Supported by the Illinois Council on Food and Agricultural Research, CF071‐006‐4‐SEN.