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A high carbohydrate diet coordinately alters transcriptomic profiles in the adipose tissue leading to enhanced lipid biosynthesis
Author(s) -
Shankar Kartik,
Harrell Amanda,
Ronis Martin J,
Badger Thomas M
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.556.2
Subject(s) - adipose tissue , fads2 , medicine , endocrinology , transcriptome , biosynthesis , glut4 , carbohydrate , fgf21 , white adipose tissue , chemistry , lipid metabolism , biology , biochemistry , gene expression , fatty acid , gene , glucose transporter , insulin , fibroblast growth factor , polyunsaturated fatty acid , docosahexaenoic acid , receptor
To assess the role of dietary macronutrient composition on adipose gene expression we evaluated changes in transcriptomic profiles in the WAT of rats following high carbohydrate (HC) diets. Female Sprague‐Dawley rats received liquid diets at 187 or 220 kcal/kg 3/4 /d via intragastric infusion. Diets were either HC (75% carb calories) or high fat (HF, 45% fat calories) and were infused 23 h/d for 3 wk. Rats fed HC diets gained greater body‐weights and %body fat (p<0.05) than HF counterparts. Microarray analyses of retroperitoneal WAT using GeneSpring Gx7.3 revealed 581 transcripts were altered by HC at either 187 or 220 Kcal (±1.8‐fold, p <0.05). Of these, 153 transcripts were altered by HC at both caloric intakes, indicating direct responsiveness to dietary carbohydrate. Specifically genes involved in glucose transport (GLUT4), glycolysis (HK2, ALDO2, Me1, ACLY, Pdh, OCD), fatty acid biosynthesis, elongation and desaturation (FASN, SCD2, ACC1, ELOVL4, 5 and 6, FADS2, adiponutrin) were significantly up‐regulated. 13 genes involved in cholesterol biosynthesis and transport were also induced by HC. Most interestingly, mRNA expression of ChRBEP was also induced in adipose tissue of HC rats. These data indicate carbohydrates transcriptionally regulate a diverse suite of genes involved in the entire pathway of lipid biosynthesis in the adipose tissue. Support ARS‐CRIS 6251‐51000‐005‐00D.