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Zinc stimulates extracellular matrix mineralization in osteoblastic MC3T3‐E1 cells
Author(s) -
Alcantara Ethel Hipolito,
Lomeda RiaAnn R,
Cho YoungEun,
Alam Md. Jahangir,
Kwun InSook
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.553.12
Subject(s) - chemistry , ascorbic acid , extracellular matrix , mineralization (soil science) , calcium , zinc , extracellular , calcification , type i collagen , osteoblast , activator (genetics) , endocrinology , medicine , biochemistry , microbiology and biotechnology , biology , in vitro , receptor , food science , organic chemistry , nitrogen
Zinc has been implicated as an activator in the regulation of bone formation. Bone formation is characterized by the synthesis of collagen, the major bone protein in the extracellullar matrix (ECM) which then becomes mineralized by calcium deposition. In this study, we investigated the effect of zinc in the mineralization of two MC3T3‐E1 subclones, SC4 and SC24,cultured in the presence and absence of ascorbic acid. Osteoblastic cells were cultured in normal osteogenic media (OSM), Zn deficient media (1 µM Zn), and Zn adequate media (15 µM Zn) with the addition of 4 µM TPEN as intracellular Zn chelator and supplemented with 75 µg/ml Vitamin C within 20 d of bone formation period. Our results showed that Zn deficiency decreased collagen synthesis and calcium deposition in both subclones. The expressions of ECM bone marker proteins (ALP, OC, OPN) which are necessary for ECM mineral deposition were also lower in zinc‐deficient osteoblasts. Although ascorbic acid is essential in the formation of collagen, Zn deficiency also decreased the synthesis of collagen even in Vit C‐rich media. These results implied that Zn stimulates mineralization by increasing collagen synthesis and calcium deposition which in turn increase expressions of ECM bone marker proteins critical for bone formation in osteoblasts. (KOSEF grant No. R01‐2006‐000‐10640‐0 and KRF grant No. KRF‐2007‐313‐C00810)