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Zinc modulation of osterix expression in osteoblastic MC3T3‐E1 cells
Author(s) -
Alam Md. Jahangir,
Cho YoungEun,
Alcantara Ethel H,
Seo HyunJu,
Kwun InSook
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.553.10
Subject(s) - runx2 , chemistry , gene expression , osteoblast , zinc , transcription factor , microbiology and biotechnology , endocrinology , gene , biology , biochemistry , in vitro , organic chemistry
Osterix is zinc finger transcription factor and downstream of Runx2, which is essential for bone formation and mineralization. In our previous study, Runx2 protein expression decreased by Zn deficiency during osteoblast differentiation phases and at 24 h, which suggests a negative regulation of Runx2. In the present study, we investigated whether Zn deficiency decreases osterix expression, and this modulation will be due to the modulation of Runx2 expression in osteoblasts. In Exp.1, osteoblastic MC3T3‐E1 cells were cultured in Zn‐dependent level (0, 0.25, 0.5, 1, 5, 15 µM ZnCl 2 ) with the cellular Zn depletion by the addition of 5 µM TPEN for 24 h. Osterix gene and protein expression was increased in a Zn concentration‐dependent manner, and Runx2 gene and protein expression showed the similar with the transition pattern of the highest Runx2 expression at 1‐5 µM Zn, which suggests Runx2 expression shifts from 15 µM Zn to the lower Zn level at 24 h. Again, to evaluate Zn effect, in Exp 2, the cells were cultured with 0‐20 µ Zn, Osterix and Runx2 gene expression showed the same pattern of Zn‐dependent. The study results suggest that osterix expression is modulating by cellular Zn level and this modulation would be due to the modulation of Runx2, which also affected by Zn in osteoblasts. (Supported by KOSEF, grant No. R01‐2006‐000‐10640‐0 and KRF grant, No. KRF‐2007‐313‐C00810)