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Effects of dietary calcium and milk on oxidative and inflammatory stress and lifespan in wild‐type and aP2‐agouti transgenic mice
Author(s) -
Bruckbauer Antje,
Sobhani Marilyn T,
Zemel Michael B
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.547.10
Subject(s) - oxidative stress , endocrinology , medicine , adipose tissue , glutathione peroxidase , biology , genetically modified mouse , wild type , transgene , chemistry , superoxide dismutase , biochemistry , gene , mutant
Dietary Ca reduces oxidative and inflammatory stress, while milk exerts a greater effect than supplemental Ca. To examine the effects on lifespan and related biomarkers, aP2‐ agouti transgenic mice (model of diet‐induced obesity) and wild‐type littermates were fed a low Ca (0.4% Ca), high‐Ca (1.2% Ca from CaCO 3 ), or milk (1.2% Ca from non‐fat dry milk) obesigenic diets until death. Randomized subgroups were sacrificed at 28, 52 and 78 weeks of age for biomarker analysis. The milk diet attenuated weight and adiposity gain and prevented the age‐related muscle loss (p<0.04) and rise in reactive oxygen production (ROS) in adipose tissue (p=0.005). There was a corresponding increase in SOD3 (in wild‐type, p=0.04) and glutathione peroxidase gene expression (p<0.02) in soleus, and in liver SOD activity (p<0.04). The milk diet decreased adipose tissue IL6 (p<0.05) and TNFα (p<0.03) expression in male mice only. The survival analysis showed an overall shorter lifespan in transgenic vs. wild‐type mice. Although no diet effect was found on lifespan, the milk diet resulted in a significant increase in early (75%) survival rate in wild‐type mice (p<0.02). These data demonstrate that milk diet attenuates adiposity, protects against muscle loss and reduces oxidative and inflammatory stress. Although these effects did not influence maximum lifespan, they did suppress early mortality. Grant Funding Source The National Dairy Council