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Effect of conjugated linoleic acid and n‐3 fatty acids on body composition and bone loss in aging C57Bl/6 mice
Author(s) -
Halade Ganesh V,
Rahman Md Mizanur,
Williams Paul,
Fernandes Gabriel
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.543.4
Subject(s) - conjugated linoleic acid , chemistry , medicine , endocrinology , steatosis , linoleic acid , corn oil , muscle hypertrophy , fatty acid , food science , biochemistry
Conjugated linoleic acid (CLA) and n‐3 fatty acids (FA) have been proposed as an important pharmaconutrient for modulating cancer and obesity. We investigated effect of CLA and n‐3 FA alone or in combination to modulate bone loss and liver steaotosis in aging C57Bl/6 mice. Twelve months old C57Bl/6 mice were fed with 10% corn oil (CO) as control, with or without 0.5 % CLA (50:50 mixture of c9t11 and t10c12 isomers), 5% n‐3 FA and a combination of 0.5 % CLA and 5% n‐3 FA for 6 months. After 6 months dietary intervention, CLA and CLA+n‐3 FA fed mice reduced body weight and maintained bone mineral density compared to CO group. Importantly, CLA induced liver hypertrophy or steatosis was completely prevented by incorporation of n‐ 3 FA in CLA diet. The liver histology revealed that lipid filled vacuoles were significantly less in n‐3 FA and CLA+n‐3 FA diet fed mice compared to CO and CLA fed mice. The inherited property of n‐3 FA to reduce triglycerides may be responsible in modulating the liver hypertrophy induced by CLA. The DXA results showed that CLA and CLA+n‐3 FA diet also increased the lean mass in the hind leg. Furthermore, femoral bone histological sections stained with oil red O showed that CLA and CLA+n‐3 FA diet prevented bone adiposity. These findings demonstrate that CLA with n‐3 FA can be used for obesity as well as osteoporosis prevention strategy. Further studies in higher species and humans are needed to confirm this observation. Research support: NIH R21 AG027562