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Effect of mixed carotenoid vs. single carotenoid supplementation on lipid peroxidation in post‐menopausal women
Author(s) -
Yeum KJ,
Yoon GA,
Yoshida Y,
Niki E,
Aldini G,
Rasmussen H,
Lian F,
Wang XD,
Russell R M
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.542.2
Subject(s) - carotenoid , lycopene , lutein , lipid peroxidation , chemistry , oxidative stress , malondialdehyde , antioxidant , food science , biochemistry , endocrinology , medicine
The effects of supplements containing individual vs. mixed carotenoids on oxidative stress were determined. Healthy, post‐menopausal women (n=37, 50‐70 yrs) were assigned to one of five groups to be supplemented with either placebo, mixed carotenoids (a combination of β‐carotene, lutein and lycopene at 4 mg/d each) or 12 mg/d of single carotenoid (β‐carotene, lutein or lycopene) in this double blind placebo‐controlled randomized study. Plasma fat‐& water‐soluble antioxidants were analyzed by HPLC‐UVD & ‐ECD, respectively; total antioxidant capacity by fluorometry; lipid peroxidation by malondialdehyde using a HPLC and by total hydroxyoctadecadienoic acids (tHODE) using a GC/MS. Although the individual carotenoid supplemented (12 mg/d) groups showed a significant increase in plasma concentration of each carotenoid supplemented (p<0.05), no significant change in any biomarker of antioxidant activity or lipid peroxidation was observed. The mixed carotenoid group showed a significant decrease in lipid peroxidation at one of two time points during treatment, as determined by linoleic acid‐adjusted tHODE (p<0.05), while the placebo group showed an increase in cholesterol‐adjusted 7‐hydroxycholesterol (p<0.05) at both time treatment points. The current study indicates oxidative stress can be modestly modulated by either a single or a mixed carotenoid supplement and whether the mixed carotenoids have an advantage remains an open question. (Supported by USDA # 58‐1950‐7‐707 & BASF Ag, Germany)