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Antibacterial And Antithrombic Activities Of Agrowastes Culture Extract Of P.Chrysogenum
Author(s) -
Mina OnyegemeOkerenta Blessing,
Ihuaku Okochi Veronica,
Albert Ebuehi O,
Uzoma Okafor Anthony
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.535.1
Subject(s) - penicillium chrysogenum , antibacterial activity , penicillin , chemistry , yeast extract , food science , bacillus subtilis , microbiology and biotechnology , fermentation , biochemistry , bacteria , antibiotics , biology , genetics
Penicillium chrysogenum is a microfungus with a potent antibiotic production activity. It is also well known for its agrowastes biodegradation and enzyme production. This study investigated the use of agro waste based culture extract of a novel P. chrysogenum PCL 501 in antibacterial and antithrombic activities (thrombin, a serine protease is involved in blood clotting). The fungus was cultivated for a period of 240 h on cassava shavings and sugar cane pulp media in submerged fermentation and the culture extract was analyzed for its antibacterial and antithrombic activities, using benzyl penicillin as reference drug. The results revealed that the two agrowastes were well utilized by the fungus for growth and the culture extract had a potent antibacterial activity against E.coli and B.subtilis . Extract from cassava shavings show potency indices of 11.76 and 10.432 against E.coli and B.subtilis respectively and that from sugarcane pulps 17.95 and 11.41 against E.coli and B.subtilis respectively. The extract inhibited thrombin (serine protease) action which explains its antithrombic activities observed in terms of delayed clotting time. A similar pattern of inhibition was also observed with the reference drug (Benzyl penicillin).