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Structure‐Dependent Cytotoxicities: C6 Glioma Cells Exposed to DNB Isomers
Author(s) -
Rivera Berenise,
Miller R. Timothy
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.533.5
Subject(s) - cytotoxicity , chemistry , viability assay , in vitro , glioma , neutral red , cell survival , microbiology and biotechnology , stereochemistry , biochemistry , cancer research , biology
Dinitrobenzene (DNB) exists as three isomers: 1,2‐, 1,3‐ and 1,4‐DNB. These DNBs are used as common intermediates in the dye and plastics industries. 1,2‐ and 1,4‐DNB cause methemeglobinemia, in contrast, 1,3‐DNB is a purported neurotoxicant in rats. The purpose of this in vitro study was to compare the relative abilities of different cytotoxicity assays to detect early changes in cell viability. C6 Glioma cells were exposed to 10‐250 µM of each DNB isomer for up to 6 hours. Cytotoxicity was then determined using MTT reduction for mitochondrial integrity, neutral red (NR) uptake for lysosomal integrity and LDH release a marker for cell death. C6 Glioma cells exhibited a concentration‐ and time‐dependent decrease in NR uptake following a four hour exposure to 1,2‐ and 1,4‐DNB with effects most evident between 50‐250 µM. However, NR uptake was the most sensitive with 1,3‐DNB exposure. Statistically significant increases in media LDH vs. total LDH after four hour exposures were observed only for 1,2‐DNB and 1,3‐DNB at 250 µM; 1,4‐DNB caused LDH leakage beginning at 100 μM, thus revealing its greater potency in this model. This project was supported in‐part by 5G12‐RR008124 from NCRR to the BBRC at UTEP and by NIEHS/NIH grant ES011982 to RTM. The views stated in this abstract are not necessarily the view of NIH/NIEHS.