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EFFECT OF PATHWAY‐INTERCONNECTORS IN SEB INDUCED APOPTOSIS RELATED EVENTS IN HUMAN PBMCS
Author(s) -
Lillge Cayla,
Zindel Kristin,
Mendis Chanaka
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.526.9
Subject(s) - peripheral blood mononuclear cell , apoptosis , inhibitory postsynaptic potential , signal transduction , microbiology and biotechnology , caspase , caspase 8 , caspase 3 , immunology , biology , chemistry , cancer research , programmed cell death , in vitro , neuroscience , genetics
Staphylococcal entrotoxin B (SEB) is one of the endotoxins that has been thoroughly investigated; however, little is known about the cascades of signaling events that explain its patho‐mechanism. This study follows a molecular route to better understand the apoptosis related events known to be induced by SEB in human peripheral blood mononuclear cells (PBMC). As SEB is known to induce multiple signal transduction pathways, we have identified a crucial pathway inter‐connector (JNK) that may have an inhibitory effect in SEB induced unwanted cellular activities. Our study was centered on inhibiting pathway inter‐connector JNK by SP600125, followed by an evaluation of the efficacy of the target through alterations to a set of known apoptosis related genes including Caspase 1, Caspase 3, Caspase 6, Caspase 7, Caspase 8, Caspase 9, SOD, and Heparanse precursor (HEP) expression patterns. We further confirmed this effect by carrying out a set of protein expression analysis. As our experimental design specifically targeted pathway inter‐connector JNK, we believe that the target is unique and has the ability to sustain longer lasting inhibitory effects. We are also confident that inhibiting such a component will have a maximum effect on a cell module, terminating the "leaking effect", which may benefit a number of experimental modules focused on disease prevention and rapid diagnostics.