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SUMO Mediated Response Pathways to Genotoxic Stress
Author(s) -
Ro Kevin,
Wohlschlegel James
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.526.8
Subject(s) - sumo protein , viability assay , microbiology and biotechnology , dna damage , cellular stress response , cell growth , chemistry , nocodazole , biology , ubiquitin , cell , biochemistry , fight or flight response , dna , gene , cytoskeleton
The different pathways undertaken by cells in response to genotoxic stress are essential to their survival. In this study, we investigated how the SUMO conjugation pathway may play an important role in responding to various environmental stresses. SUMO is a member of the ubiquitin family of small protein modifiers and its covalent attachment to cellular targets is a critical regulatory step for a diverse range of cellular processes that includes transcriptional regulation, cell cycle progression, and DNA damage response. Specifically, we analyzed how the cell's viability and SUMO conjugation patterns changed when exposed to both acute and long term treatments of ethanol, hydrogen peroxide, hydroxyurea, nocodazole, and benomyl. To study how different stresses affected SUMO conjugation pathway, and subsequently cell viability, yeast strains deficient in key SUMO conjugation enzymes were knocked‐out and subjected to the stresses. Analysis using Western blots and growth assays reveal that several of the stresses used increased SUMOylation and affects cell growth. Proteomic analysis using a linear ion trap‐orbitrap hybrid mass spectrometer was also done on the ethanol subset and 119 SUMO substrates were found to be present in greater abundance in ethanol treated cells versus untreated cells. We are currently further characterizing the functional role of this set of SUMO targets in the cellular stress response.