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Localizing compartmental gene expression in developing mouse prostate
Author(s) -
Frank Sander,
Chen Qian,
Sikes Robert
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.515.1
Subject(s) - prostate cancer , biology , prostate , gene , complementary dna , genitourinary system , mesenchyme , cdna library , gene expression , microbiology and biotechnology , messenger rna , computational biology , epithelium , cancer research , pathology , cancer , anatomy , genetics , medicine
The prostate gland is a significant source of male genitourinary tract morbidity. As developmental processes share several features in common with metastatic cancer, we hypothesize that a better understanding of genes involved in prostate morphogenesis may identify possible targets for therapeutic intervention. The prostate is derived from the urogenital sinus (UGS) which is composed of 4 subdomains: epithelium/mesenchyme (UGE/UGM) and dorsal/ventral halves (UGD/UGV). Each region develops into specific lobes of the prostate with unique properties. This study sought to validate prostate developmental cDNA arrays that showed about 530 UGE/UGM and 35 UGD/UGV differentiated genes. Male UGS were separated either into UGE/UGM or UGD/UGV. Total RNA was extracted and used to synthesize cDNA templates for each subdomain. Quantitative‐polymerase chain reaction (Q‐PCR) was performed to observe relative mRNA levels and confirmed localization for 10/12 primary and 6/10 secondary compartmental genes. Immunofluorescence (IF) localized 6 genes in different predicted compartments. With this research, we have established the basis for a map of regional gene expression in the murine UGS. This work identifies candidate genes for further study and functional analysis by other developmental laboratories. Funding: NIH DK63919 and P20RR016472. Individual support: UD science and engineering and Stetson awards.