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Evaluation of docosahexaenoic and arachidonic acid as modifiers of carnitine palmitoyltransferase in pre‐ and term pigs
Author(s) -
Martin Jessica E,
Campbell Jenny A,
Brockson M. Elizabeth,
Melendez Krizia,
Lyvers Peffer Pasha A
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.506.4
Subject(s) - docosahexaenoic acid , carnitine , arachidonic acid , medicine , endocrinology , gestation , downregulation and upregulation , concomitant , chemistry , biology , fatty acid , polyunsaturated fatty acid , biochemistry , pregnancy , enzyme , genetics , gene
Pigs were delivered by cesarean at 106 or 114d of gestation and fitted with umbilical catheters. Pigs received either a control, a low DHA/AA (0.3 and 0.6% of total lipids as DHA and AA, respectively), or a high DHA/AA (5% and 11 % of total lipids as DHA and AA, respectively) total parenteral diet for 6d. Hepatic CPT activity was measured before and after malonyl‐CoA inhibition. There was a tendency for increased total CPT activity in preterm compared to term pigs receiving the control diet (p = 0.07) and was attributed to malonyl‐CoA insensitive activity, which was elevated across dietary treatments in preterm versus term pigs (p<0.05). This increased CPT activity was concomitant with increased CPT I transcript amounts (p < 0.05). Whereas the low DHA/AA diet resulted in postnatal upregulation of hepatic CPT I mRNA in term pigs, the control and high DHA/AA diet elicited a similar response in CPT I mRNA in preterm pigs (p < 0.05). It is unlikely that the differences observed in CPT can be ascribed to PPARα as transcript amounts were increased in term pigs receiving the control and low DHA/AA diets (p < 0.05), yet postnatal changes in PPARα were not observed in preterm pigs receiving the high DHA/AA diet. Collectively, these findings suggest that postnatal induction of hepatic CPT I is more robust in preterm pigs. This research has been supported by funds appropriated by the Ohio General Assembly to The Ohio State University, Ohio Agricultural Research and Development Center.