Premium
Role of melatonin as essential factor of growth and proliferation in SCN cells
Author(s) -
Hong Yonggeun,
Lee Sangkil,
Lee Youngjeon,
Kim Jooheon
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.491.7
Subject(s) - melatonin , cell growth , medicine , endocrinology , cell cycle , chemistry , cell , biology , biochemistry
This study was investigated to explore the effects of melatonin(N‐acetyl‐5‐methoxytryptamine) on cell proliferation and growth of SCN(suprachiasmatic nucleus) cells. The cells were cultured under 1% FBS, then treated various concentration of melatonin(10 nM, 100 nM, 1 uM, 10 uM) at indicated time points during 48 hr. Higher concentrations of melatonin(10 uM, and 1 uM) considerably inhibited the cell growth. We also found that melatonin at high concentrations(10 uM) suppressed SCN proliferation but not at low concentrations(10 nM). The cell numbers were significantly increased at 10 nM melatonin treatment(P<0.01). Furthermore, using FACS, melatonin treated cells were analyzed cell cycle, showing significantly increased the G1 phase(P<0.05) but showing decrease of S phase(P<0.05). These results indicate that melatonin induced cell proliferation and growth of SCN cells, and which was maximized at 10 nM of melatonin treated. Supported by BioGreen 21 Program(#20070401034006) from Rural development Administration, South Korea