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Antiproliferative Effects of Novel Glycosylated 24‐kDa Human Growth Hormone on MCF‐7 Breast Cancer Cells
Author(s) -
Haro Luis S.,
Haro Elizabeth K.,
Muñoz Jesus,
Martinez Andrew O.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.491.11
Subject(s) - mcf 7 , breast cancer , cell growth , endocrinology , medicine , growth inhibition , cancer cell , cancer , cancer research , in vivo , in vitro , human breast , chemistry , biology , biochemistry , microbiology and biotechnology
Human growth hormone (hGH) has been correlated with increased incidence of breast cancer, pointing to a need for development of hGH antagonists to block hGH‐dependent breast cancers. The objective of this research was to investigate the new untested concept that glycosylated 24‐kDa‐hGH would impede proliferation of human breast cancer cells. The hypothesis tested was that glycosylated 24‐kDa‐hGH would produce growth‐inhibitory effects in MCF‐7 human breast cancer cells. Methods To test this hypothesis, the number of viable breast cancer cells were quantified following a 72 hr incubation in nutrient‐rich media at 37 °C, in a humidified atmosphere of 95% air, 5% CO2, in the absence of GHs (vehicle control) or in the presence of either 22‐kDa hGH or glycosylated 24‐kDa‐hGH. One‐Way ANOVA followed by Student‐Neuman‐Keuls post‐hoc test assessed differences in breast cancer cell proliferation among the treatments. Results demonstrated that glycosylated 24‐kDa‐hGH significantly decreased the growth of human breast cancer cells compared to the 22‐kDa hGH and to the control group whereas 22‐kDa hGH stimulated their proliferation. Glycosylated 24‐kDa‐hGH acted as an antagonist of 22‐kDa hGH. The importance of this work is that it demonstrates that glycosylated 24‐kDa‐hGH possesses therapeutic potential as an anti‐neoplastic agent for combating human breast cancer growth in vivo.