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Interaction of the Glucocorticoid Receptor with the Chromatin Landscape
Author(s) -
Hager Gordon Lee,
Sabo Pete,
Thurman Robert,
Stamatoyannopoulos John A,
Sung MyongHee,
Biddie Simon C,
John Sam
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.487.3
Subject(s) - chromatin , chromatin remodeling , chia pet , biology , glucocorticoid receptor , locus (genetics) , gene , transcription factor , genome , genetics , scaffold/matrix attachment region , microbiology and biotechnology , nuclear receptor , receptor
In model systems, nuclear receptors induce chromatin remodeling at regulatory elements. We have carried out a genome‐wide analysis of GR binding events, as well as a global characterization of DNaseI hypersensitive transitions associated with GR responsive genes. Our findings indicate that receptor/genome interactions are universally associated with local chromatin remodeling events. Furthermore, multiple remodeling systems are required for these events. The presence of these locally open chromatin regions is highly cell specific, and both GR binding and GR transcription regulation correlate well with the presence of these sites. These results indicate that the chromatin status of a specific gene locus in a given cell type is a critical determinant for the GR responsiveness of the locus.