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The 19S proteasome positively regulates the chromatin structure of cytokine inducible genes
Author(s) -
Koues Olivia I,
Dudley Robert Kyle,
Greer Susanna F
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.487.2
Subject(s) - histone , biology , chromatin remodeling , microbiology and biotechnology , histone methylation , chromatin immunoprecipitation , histone h2a , histone methyltransferase , proteasome , chromatin , histone modifying enzymes , histone h2b , promoter , genetics , gene , gene expression , dna methylation
Recent studies have indicated that the 19S proteasome's function extends beyond that of a role in protein degradation to an important role in the regulation of transcription. Research in yeast shows that 19S ATPases associate with active promoters and recruit the histone modifying SAGA complex. Also, components of the 19S proteasome are known to directly link histone activating events including histone H2B ubiquitination and histone H3 methylation. Although 19S ATPases facilitate these and other chromatin remodeling events in yeast, it was less clear until recently if they play similar roles in mammalian cells. We have shown that 19S ATPases positively regulate histone acetylation at mammalian inflammatory genes. Here we further characterize the role the 19S proteasome plays in regulating and coordinating additional activating histone modifications, including enzyme recruitment and histone methylation and ubiquitination. Our current study emphasizes the importance of the 19S proteasome in modifying histones to initiate transcription of cytokine inducible genes and provides novel insights into the regulation of mammalian transcription. Research supported by grants from the American Cancer Society, the Georgia Cancer Coalition, the Georgia Research Alliance, and Georgia State University.