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The Cystatin S Promoter Drives Expression in the Spleen, Brain and Lung
Author(s) -
Shaw Phyllis A.,
Li Jinhua,
Luo Jian
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.470.5
Subject(s) - spleen , biology , messenger rna , transgene , green fluorescent protein , salivary gland , microbiology and biotechnology , lung , kidney , gene expression , gene , genetically modified mouse , medicine , endocrinology , immunology , biochemistry
The cystatin S gene has 3 types of transcription factor binding "modules" clustered in its 1.9kb 5′‐flanking region, including a hormone module containing 3 regions found in the 5′‐flanking sequence of all known salivary gland specific genes, an immune module and 2 SRY (male sex determining modules). To determine which of the cis ‐elements regulate tissue specific expression of the cystatin S gene, we generated 2 transgenic mouse models. The 1.9kb/GFP expressing transgene, contains all 4 modules and salivary gland‐specific elements; the 1kb/GFP expressing transgene has the hormone, an SRY and immune modules, and salivary gland‐specific regions. We examined 1.9kb and 1kb promoter driven expression of GFP mRNA and GFP protein levels during development of the submandibular, sublingual, and parotid glands, liver, heart, kidney, muscle, spleen, brain, and lung. 1.9kb of the cystatin S promoter regulates GFP mRNA in all tissues examined during development. Submandibular, sublingual and parotid glands exhibit the highest levels of GFP mRNA at days 10 and 20; the liver, heart, muscle, kidney, brain and lung have the highest level of GFP mRNA at day 10 of development. Spleen shows the highest level of GFP mRNA at day 25. Spleen, kidney, brain and lung have high levels of GFP protein in the 1.9kb/GFP developing transgenic mice. GFP protein remains high in spleen, brain and lung, but is not detected in kidneys of 1kb/GFP transgenics.