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Mouse Latissimus Dorsi as a model system for evaluating tissue engineered skeletal muscle
Author(s) -
Machingal Masood A.,
Vishwajit Sonia,
Bishwokarma Bimjhana,
Zhao Weixin,
Yoo James J,
Christ George J.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.468.4
Subject(s) - skeletal muscle , decellularization , in vivo , isometric exercise , latissimus dorsi muscle , matrix (chemical analysis) , tissue engineering , biomedical engineering , anatomy , chemistry , medicine , biology , microbiology and biotechnology , chromatography
The inability to engineer clinically relevant functional muscle tissues in vitro remains a major obstacle to the development of successful procedures for skeletal muscle (SKM) replacement in vivo. The goal of these studies is to further develop the technologies required for the creation of skeletal muscle biomimetics that can be used clinically for restoration of muscle function in vivo. As a first step in this direction we have examined the rodent latissimus dorsi (LD) as a model system for testing functional replacement of native SKM with tissue engineered SKM constructs (TE‐SKM). Porcine bladder acellular matrix was prepared and rat muscle precursor cells were seeded onto the matrix and preconditioned in a custom designed, computer controlled cyclic strain bioreactor for one week prior to subcutaneous implantation in nu/nu mice onto LD for 1 month. The size of the TE‐SKM was 15 mm X 3mm X 0.35 mm; dimensions similar to that of the native mouse LD. TE‐SKM isolated after one month implantation showed a maximal isometric tetanic response of 42.9 mN/mm2 which was 26 % of that of native LD isolated from contra lateral side, but 50‐ and 3500‐fold greater than that previously observed for TE‐SKM. To our knowledge, this is the largest specific force yet recorded for TE‐SKM on a decellularized matrix. In short, the LD appears to be an attractive model for further studies of functional SKM replacement with TE‐SKM constructs.