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The development of biomaterials from porcine skeletal muscle extracellular matrix
Author(s) -
Stern Matthew Michael,
Fearing Bailey V.,
Soker Shay,
Kritchevsky Stephen B.,
Christ George J.,
Van Dyke Mark
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.468.1
Subject(s) - decellularization , extracellular matrix , skeletal muscle , tissue engineering , scaffold , regenerative medicine , myocyte , chemistry , regeneration (biology) , microbiology and biotechnology , biomedical engineering , anatomy , cell , biochemistry , biology , medicine
There is currently great interest in the development of biomaterials for use in the engineering and/or regeneration of skeletal muscle tissue. Here, we describe the development of an acellular scaffolding system from porcine skeletal muscle extracellular matrix (ECM) molecules. Acellular scaffolds were prepared through the decellularization, lyophilization, and sterilization of thin slices of porcine loin muscle tissue. Myogenic cells seeded onto these scaffolds exhibited the ability to fully penetrate and recellularize the scaffolds. This represents a significant advance over scaffolding systems in which seeded cells fail to penetrate and simply pile onto the scaffold's surface. We also demonstrate that decellularized skeletal muscle tissue can be processed into bioactive powder or gel forms that promote the growth of myogenic cells. Gels composed of skeletal muscle ECM embedded with myogenic cells supported cell growth and gel remodeling. Myogenic cells cultured on surfaces coated with skeletal muscle ECM extract underwent enhanced proliferation relative to uncoated surfaces and simple collagen I coated surfaces. These results demonstrate the feasibility of using bioactive materials comprised of porcine skeletal muscle ECM molecules to produce different classes of biomaterials for use in tissue engineering and regenerative medicine applications.