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Identification of regenerating myofibers within an extracellular matrix seeded with bone marrow derived cells
Author(s) -
Tierney Matthew,
Merritt Edward,
Sarathy Apurva,
Walters Thomas,
Farrar Roger
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.467.5
Subject(s) - extracellular matrix , myogenin , microbiology and biotechnology , desmin , matrix (chemical analysis) , tissue engineering , muscle tissue , myocyte , regenerative medicine , bone marrow , chemistry , anatomy , biology , biomedical engineering , immunohistochemistry , stem cell , medicine , immunology , myogenesis , vimentin , chromatography
Despite the normally robust regenerative capacity of muscle tissue, extensive soft tissue damage often results in a functional loss that cannot be restored using classic reconstruction techniques. Although implanted biomaterials may be capable of mechanically transmitting force generated from the remaining tissue, cellular repopulation of the damaged area is necessary to achieve complete functional restoration. Using an in vivo tissue engineering model, a 1 x 1 cm portion of the lateral gastrocnemius (LGAS) of Lewis rats was removed and replaced with a muscle‐derived extracellular matrix (ECM). Bone marrow‐derived cells (BMCs) were injected within the ECM 7 days post‐injury. Immunohistochemical identification of desmin, a structural muscle protein, and myogenin, a late transcription factor required for myoblast differentiation, was performed within the defected area. Regenerating myofibers as identified by these markers were visually identified within the ECM following 28 & 42 days recovery. These results demonstrate the ability of ECM to support cellular ingrowth in this model. This work was funded in part by the US Army MRMC Grant DAMD W81XWH‐06‐1‐0540.