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The Role of Hepatocyte Growth Factor in Satellite Cell Mediated Angiogenesis
Author(s) -
Flann Kyle L.,
Rathbone Chris R.,
Allen Ron E.,
Rhoads Robert P.
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.467.3
Subject(s) - hepatocyte growth factor , angiogenesis , satellite , microbiology and biotechnology , cell growth , growth factor , cell , cell culture , biology , chemistry , cancer research , biochemistry , receptor , genetics , engineering , aerospace engineering
Skeletal muscle regeneration is a multifaceted process involving the spatial and temporal coordination of myogenic and angiogenic events. Satellite cells are well‐established myogenic precursor cells responsible for skeletal muscle growth and repair. We have recently demonstrated that satellite cells also promote angiogenesis, in part, through the secretion of soluble‐acting factors. Satellite cells produce a number of growth factors, some with pro‐angiogenic properties, although it not clear which of these growth factors participate in satellite cell‐mediated angiogenesis. One likely candidate protein is hepatocyte growth factor (HGF). To identify a role for HGF in satellite cell mediated angiogenesis, we employed an in vitro model consisting of rat microvascular fragments (MVF) embedded in a three‐dimensional collagen matrix. In an effort to block HGF action, various levels of anti‐HGF antibody were added to rat satellite cell conditioned medium and fed to MVF cultures. Following 5 days of culture, MVF were analyzed for indices of angiogenesis. Sprout number and length were decreased in a dose‐dependent manner by increasing levels of HGF inhibition. Addition of recombinant HGF during HGF inhibition sufficiently rescued angiogenesis in the MVF cultures. This data indicates that satellite cell‐derived HGF plays an important role in satellite cell mediated angiogenic processes. Grant Funding Source NIH