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Primitive stem cells in adult feline, canine, ovine, caprine, bovine, and equine peripheral blood
Author(s) -
Lochner F,
Lochner D,
Lochner D,
Samples O,
McCommon G,
Alena C,
Hixson D,
Bowyer F P,
Black A C,
Young H E
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.466.5
Subject(s) - trypan blue , hemocytometer , peripheral blood , cats , andrology , stem cell , biology , vacutainer , medicine , pathology , immunology , microbiology and biotechnology , cell , biochemistry
Stout et al. (Amer Surg. 73:1106‐1110, 2007) reported primitive endogenous stem cells circulating within adult porcine peripheral blood. The current study was undertaken to determine whether similar primitive stem cells could be isolated from the peripheral blood of adult felines, canines, ovines, caprines, bovines, and equines. Adult cats, dogs, sheep, goats, cows and horses had their blood withdrawn following the guidelines of FVSU‐IACUC. The blood was obtained by venipuncture and four ml withdrawn. After gravity separation, the plasma fraction was withdrawn and 15 microliters of the plasma from each animal was mixed with 15 microliters of 0.4% Trypan blue. The solution was mixed, placed onto a hemocytometer and the isolated cells counted: blastomere‐like stem cells (BLSCs) are trypan blue positive and < 2.0 microns in size; transitional‐BLSCs (Tr‐BLSCs) are both trypan blue positive & negative and 2‐5 microns in size; and epiblast‐like stem cells (ELSCs) are trypan blue negative and 6‐8 microns in size (Young and Black, Minerva Biotech 17:55‐63, 2005) . The results show that BLSCs, Tr‐BLSCs, and ELSCs are circulating within the peripheral blood of all species examined.