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Amniotic Fluid‐derived Stem Cells For Regeneration of Infracted Rat Myocardium
Author(s) -
Guan Xuan,
Delo Dawn,
Wang Zhan,
Shaffer Robyn,
Groban Leanne,
Callahan Michael,
Smith Tom,
D'Agostino Ralph B.,
Atala Anthony,
Soker Shay
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.465.7
Subject(s) - cardiac function curve , stem cell , cardiac marker , immunostaining , myocardial infarction , myocyte , medicine , troponin , troponin i , cardiology , chemistry , microbiology and biotechnology , biology , immunohistochemistry , heart failure
Objective This experiment was set to determine if human amniotic fluid‐derived stem (hAFS) cells could be differentiated into cardiac lineage, engraft into infarcted myocardium and affect heart function. Methods AFS cells were treated with 5‐aza‐2‐deoxycytidine and analyzed for expression of cardiac markers by PCR and immunostaining. For in vivo study, 5 million cells were injected into hearts immediately after infarction. Heart function was monitered by echocardiography and invasive hemodynamic study. All hearts were analyzed 3 months after injection. Results Differentiated cells expressed cardiac Troponin I, Troponin T, Myosin light chain 2v, MEF2C, MEF2D and Nkx2.5. Undifferentiated and differentiated hAFS cells engraft into infarcted hearts. When compared to control, injection of undifferentiated cells reduced left ventricular end systolic diameter (p=0.098) and improving fractional shortening (p=0.074) while differentiated cells had opposite effect (p = 0.017 and 0.01). Conclusions The result indicates that hAFS cells can be differentiated into cardiac phenotype, engraft into infarcted myocardium and affecting cardiac function. Undifferentiated hAFS cells tend to improve cardiac function while differentiated cells worsen function. The reason for this difference remains unclear.