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Comparative expression and transcriptional networks of human‐mouse syntenic genes during osteoblastic differentiation of bone marrow mesenchymal stem cells
Author(s) -
Dernowsek Janaina Andréa,
Fontana Vanessa,
Evangelista Adriane F,
Junta Cristina M,
Marques Márcia M C,
Macedo Claudia,
Simões Belinda P,
Prado Karina F B,
Passos Geraldo A S
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.465.5
Subject(s) - biology , synteny , gene expression profiling , gene , mesenchymal stem cell , gene expression , complementary dna , microarray analysis techniques , microbiology and biotechnology , genetics , genome
Osteoblasts are cells differentiate from mesenchymal precursors in a complex process regulated by transcription factors that regulate the expression of key genes. Many of these genes present human‐mouse synteny, i.e, they share sequence identity and the same physical distribution in chromosomal regions. Our aim in this study was to compare the transcriptional profiling and network interactions of syntenic genes during the osteogenesis in vitro . The mesenchymal stem cell (MSC) were collected from of the bone marrow of adult healthy donors or of 4 week‐old mice. SC cells were cultured in α‐MEM medium with fetal bovine serum. After expansion, cells were transferred to differentiation medium containing. Total RNA was prepared from undifferentiated and differentiated MSC after 24 and 48 h and 7 days. Fluorescent Cy3/Cy5 cDNA probes from total RNA of these cells were generated and hybridized with a cDNA microarray. The gene expression profiling was analyzed by hierarchical clustering and to reconstruct the regulatory transcriptional networks, we used the GeneNetwork program. A set of 227 syntenic genes were identified from which 108 were comodulated, i.e, genes presenting the same expression profiling. Gene profiling, although useful for characterizing the osteogenesis and identifying the expression signatures of syntenic genes. The GeneNetwork program was a useful tool allowing the detection of seven syntenic gene nodes ( SHMT1, CACNB3, CASP8, GFM2, XPC, SYNGR2 and SDF2), which establish interactions with other genes. These are then considered as candidate genes controlling the osteogenesis. Grant Funding Source FAPESP, CNPQ, FAEPA