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Murine epidermal progenitor cells have a low redox state
Author(s) -
Carr Wanakee,
Zhang Yuping,
Oberley Christopher,
Oberley Larry W,
Dunnwald Martine
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.465.1
Subject(s) - microbiology and biotechnology , epidermis (zoology) , catalase , reactive oxygen species , progenitor cell , biology , oxidative stress , population , stem cell , intracellular , cellular differentiation , chemistry , biochemistry , anatomy , demography , sociology , gene
The epidermis is a stratified cornified epithelium that protects the organism against physical and microbial insults. These environmental exposures can result in oxidative stress and affect cellular proliferation and differentiation. However, very few studies have evaluated the base line levels of reactive oxygen species (ROS) and antioxidant proteins in the epidermis or in the context of stem/progenitor cells, cellular types critical to the homeostasis of the epidermis. Here, we show that ROS are mainly produced by epidermal cells and that antioxidant proteins (MnSOD, GPx1, catalase) are differentially expressed in the epidermis. Using our previously sorted epidermal progenitor cells (side population, EpSPs) and more differentiated cells (non‐SPs), we show that catalase expression and activity are significantly decreased in EpSPs compared to non‐SPs. Furthermore, we found fewer peroxisomes per cell and decreased mitochondrial surface area in the EpSPs compared to non‐SPs. Using dihydroethidium (DHE), we show that EpSPs have less DHE signal compared to more differentiated cells. In summary, our data suggest that EpSPs have decreased levels of antioxidant proteins and ROS. Together, these studies indicate that the intracellular redox environment of EpSPs is reduced relative to their more differentiated progeny. Grant Funding Source National Institute of Health