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Chromatin signatures in multipotent hematopoietic stem cells indicate fate of bivalent genes during differentiation
Author(s) -
Zhao Keji
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.421.2
Subject(s) - stem cell , biology , chromatin , bivalent (engine) , haematopoiesis , cellular differentiation , multipotent stem cell , histone , microbiology and biotechnology , progenitor cell , bivalent chromatin , genetics , gene , chromatin remodeling , chemistry , organic chemistry , metal
Histone modifications are implicated in the maintenance and differentiation of stem cells. We analyzed genome‐wide changes of multiple histone modifications during differentiation of multipotent hematopoietic stem/progenitor cells (HSCs/HPCs) into erythrocyte precursor cells. We find that several monomethylations associate with critical enhancers of differentiation genes prior to their activation and are correlated with their basal level expression, suggesting that they are involved in the maintenance of the activation potential required for differentiation. The bivalent genes that lose H3K27me3 after differentiation are associated with increased levels of H2A.Z, H3K4me1, H3K9me1, H4K20me1 and Pol II in HSCs/HPCs, suggesting that the fate of bivalent genes during differentiation is already programmed by chromatin modifications at the HSC/HPC stage.