Premium
Cellular Senescence and Remodeling of Adult Ventricular Myocyte (AVM) in Primary Culture
Author(s) -
Liu Shi Jesse,
Liu MeeiYueh G
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.420.5
Subject(s) - senescence , propidium iodide , apoptosis , medicine , programmed cell death , myocyte , necrosis , muscle hypertrophy , pathology , biology , endocrinology , microbiology and biotechnology , biochemistry
AVM were considered terminally differentiated, which limits recovery after injury. AVM death during cardiac aging was suggested to involve cellular hypertrophy, apoptosis and necrosis. In vitro studies of AVM were limited by a short average life span in culture (~4 weeks). Additionally, the temporal changes in individual AVM during senescence have not been characterized. Thus, this study is to investigate cellular senescence in primary culture of AVM, isolated from young adult rat hearts. AVM were maintained in antibiotic‐ and antimycotic‐free M199 culture medium with 2% fetal bovine serum (refreshed every 4‐6 days) in an incubator with 5% CO 2 and 95% air at 37°C for 15‐20 weeks. The growth of AVM was monitored with video microscopy and characterized with immunofluorescence staining of cardiac troponin I, connexin 43, atrial natriuretic peptide and AnnexinV+ propidium iodide in some experiments. Results showed that AVM retained myofilaments with spontaneous contracting activity and underwent hypertrophy and continuously remodeling for cell‐cell contact to form contracting fibers. Some AVM were able to regenerate to repair injury and reproduce occasionally new cells throughout the culture period. Furthermore, AVM death during senescence was inconsistent with apoptosis. In conclusion, AVM in long‐term culture can be valuable to studies of cardiac cell senescence. (Supported by UAMS departmental funding)