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Stem Cell Transplantation for Cardiac Repair is Improved by Mechanical Preconditioning
Author(s) -
Drowley Lauren,
Cassino Theresa,
Okada Masaho,
Keller Bradley,
Tobita Kimimasa,
LeDuc Philip,
Huard Johnny
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.362.9
Subject(s) - angiogenesis , transplantation , secretion , myocardial infarction , cardiac function curve , myocyte , vascular endothelial growth factor , medicine , regeneration (biology) , stem cell , stimulation , endothelial stem cell , cardiology , cancer research , microbiology and biotechnology , chemistry , in vitro , vegf receptors , biology , heart failure , biochemistry
We have previously shown that muscle‐derived stem cells (MDSCs) are significantly better than myoblasts at maintaining cardiac function after a myocardial infarction, which is likely due to higher survival and vascular endothelial growth factor secretion (VEGF) after transplantation. VEGF has been shown to be critical for cardiac repair, and mechanically stimulated MDSCs have increased VEGF secretion. We hypothesized that transplanting mechanically preconditioned MDSCs would result in increased repair of a myocardial infarction. After injection with mechanically stretched MDSCs, cardiac function at all time points is improved above unstimulated cells and saline. The improvement is likely due to an increase in angiogenesis and a small decrease in scar tissue formation in the mechanically preconditioned group. To clarify the mechanisms we explored cell behavior in vitro, where we saw increased VEGF secretion. Results from our VEGF secretion study also demonstrate that the effect of mechanical stimulation is time‐dependent, indicating that loading history control may be important to achieve ideal regeneration. The tight regulation and timing demonstrated by these results suggest that the mechanical loading history can predispose the MSDCs toward specific responses that are advantageous for cell transplantation.