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The Role of microRNAs in Liver Regeneration in Rats
Author(s) -
Paranjpe Shirish,
Bowen William,
Michalopoulos George K,
LaFramboise William A
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.361.6
Subject(s) - microrna , biology , liver regeneration , gene , endogeny , gene expression , gene expression profiling , microarray , microbiology and biotechnology , microarray analysis techniques , andrology , regeneration (biology) , genetics , endocrinology , medicine
The discovery of endogenous small (18 ‐ 25 nucleotide) noncoding microRNAs (miRNA) as powerful negative regulators of gene expression has had an enormous impact in biology. It has been estimated that miRNAs regulate about 30% of genes in humans, with an average of 200 mRNA targets/microRNA. Liver regeneration after a two‐thirds partial hepatectomy (PHx) is an extremely complex process requiring interaction and cooperation of many growth factors and cytokines and crosstalk between multiple pathways. To our knowledge, not much is known about the role of miRNAs in liver regeneration in rats following a PHx. MiRNA profiling in regenerating rat liver at various time points post PHx, post sham operation and resting rat liver was carried out. Microarray data of profile obtained at one‐hour was analyzed by ANOVA to test for independent effects of time versus PHx and control groups for differentially expressed miRNA. Preliminary analysis of microRNA profile in control and regenerating liver indicated that 28 miRNAs were significantly regulated in the PHx group as compared to resting liver and sham control. The miRNAs of let‐7 family were significantly down regulated and was confirmed by RT‐PCR. In conclusion, miRNAs predicted to target genes associated with cellular proliferation, transcription factors and apoptosis were significantly regulated with respect to both class (sham operated controls and PHx) and time post PHx.