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Expression of Aquaporin 4 mRNA during Acute Stage of Traumatic Brain Injury in Rats
Author(s) -
Hao chunyan,
Duan hubin,
Li shuzhen
Publication year - 2009
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.23.1_supplement.356.7
Subject(s) - traumatic brain injury , aquaporin 4 , brain edema , edema , messenger rna , medicine , endocrinology , anesthesia , pathology , biology , gene , biochemistry , psychiatry
Backgroud and purpose The aquaporin 4 (AQP4) water channel is abundant in rats brain and this protein plays a major role in the control of water flow within the central nervous system. This study is to investigate the expression of AQP4 mRNA and its correlation with brain edema during the acute stage of traumatic brain injury (TBI) in rats. Methods 56 adult male Wistar rats were randomized into the TBI group and control group.TBI group were again stratified into 7 sub‐groups according to the time after injury (0.5,2,6,12,24,48 and 72 h).Severe injury models (2.5‐3.0atm ) was produced with the fluid percussion brain injury (FPI) technique in anesthetized adult rats equipped with a closed cranial window.At each time spots, the water contents in the brain tissues were measured by wet‐to‐dry weight ratio and the expression of AQP4 mRNA was assessed by semi‐quantitative RT‐PCR. Results The expression of AQP4 mRNA was up‐regulated 0.5 hours after injury,progressively enhanced from 2h to12 h, peaked at 24 h(P<0.05) and remained a high level at 72 h.A similar change was noted for water contents in brain tissues. The expression of AQP4 mRNA was positively correlated with brain water contents(r=0.095,P<0.05). Conclusion The changes in AQP4 mRNA expression may be closely related to formation and progression of brain edema during the acute stage of severe traumatic brain injury.AQP4 may be playing an important role in brain edema after severe TBI.